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NNMT inhibitors display high membrane permeability Compounds spanning ~100-fold IC 50 values for NNMT inhibition were selected on the basis of positional substitutions around the N-methylated quinolinium scaffold[17] to obtain an estimate of drug-like oral absorption/bioavailability properties and guide the choice of inhibitors for in vitro and in vivo phenotypic studies

Nakamoto M, Kashiwagi Y, Yamamoto M (2005) Synthesis and size regulation of gold nanoparticles by controlled thermolysis of ammonium gold(I) thiolate in the absence or presence of amines

Sekhar RV, McKay SV, Patel SG, Guthikonda AP, Reddy VT, Balasubramanyam A, Jahoor F

Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step